Syntaxin 16
A SNARE protein involved in trans-Golgi network trafficking
Fact Sheet
| Monoclonal mouse antibody | |
|---|---|
|
Cat. No. 110 161 IgG, unlabeled |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: yes ICC: not recommended IHC: not recommended ELISA: yes (see remarks) |
| Clone | 148.6 |
| Subtype | IgG2b |
| Immunogen | Recombinant protein of rat syntaxin 16b (aa 1 - 302). |
| Reactivity | Rat; other species not tested yet. |
| Specificity | Specific for syntaxin 16. |
| Remarks | ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 110 163 as detector antibody (protocol for sandwich-ELISA). |
| Polyclonal rabbit antibody | |
|
Cat. No. 110 162 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 110 163 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 5000 (AP staining) 110 162 110 163
IP: serum: no, purified antibody: not tested yet ICC: 1 : 500 110 162 110 163 IHC: 1 : 2000 (see remarks) 110 162 (only serum tested yet) ELISA: yes (cat. no. 110 163; see remarks) |
| Immunogen | Recombinant protein of rat syntaxin 16b (aa 1 - 302). |
| Reactivity | Human, rat, mouse, hamster. |
| Specificity | Recognizes all four isoforms syntaxin 16a, b, c, d. |
| Remarks |
IHC: Works well with paraffin embedded material. ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 110 161 as capture antibody (protocol for sandwich-ELISA). |
| Control protein | |
| Cat. No. 110-16P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the recombinant protein (aa 1 - 302) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
