Syntaxin 16
A SNARE protein involved in trans-Golgi network trafficking
Fact Sheet Cat. No. 110 162, 110 163
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 110 162 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 110 163 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 5000 (AP staining) 110 162 110 163
IP: serum: no, purified antibody: not tested yet ICC: 1 : 500 110 162 110 163 IHC: 1 : 2000 (see remarks) 110 162 (only serum tested yet) ELISA: yes (cat. no. 110 163; see remarks) |
| Immunogen | Recombinant protein of rat syntaxin 16b (aa 1 - 302). |
| Reactivity | Human, rat, mouse, hamster. |
| Specificity | Recognizes all four isoforms syntaxin 16a, b, c, d. |
| Remarks |
IHC: Works well with paraffin embedded material. ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 110 161 as capture antibody (protocol for sandwich-ELISA). |
