Syntaxin 12/13

A syntaxin family member functioning in the recycling of plasma membrane receptors

Fact Sheet

Monoclonal mouse antibody
Cat. No. 110 131 IgG, unlabeled	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: 1 : 100 up to 1 : 500 image IHC: not tested yet
Clone	151.2
Subtype	IgG1
Immunogen	Recombinant cytoplasmic domain of rat syntaxin 13 (aa 1 - 250).
Reactivity	Rat; other species not tested yet.
Specificity	Specific for syntaxin 12/13.

Polyclonal rabbit antibody
Cat. No. 110 132 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Cat. No. 110 133 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 100 up to 1 : 1000 (AP staining) 110 132 110 133 IP: yes (only serum tested yet) ICC: 1 : 100 up to 1 : 500 110 132 110 133 IHC: 1 : 500 (see remarks) 110 132 (only serum tested yet)
Immunogen	Recombinant cytoplasmic domain of rat syntaxin 13 (aa 1 - 250).
Reactivity	Human, rat, mouse, hamster, chicken.
Specificity	Specific for syntaxin 12/13.
Remarks	IHC: Works well with paraffin embedded material.

Control protein
Cat. No. 110-13P	100 µg lyophilized protein. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of protein in TBS. Then aliquot and store at -20°C until use.
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control protein consists of the cytoplasmic domain of rat syntaxin 13 that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.