Syntaxin 1
Plasma membrane protein essential for synaptic vesicle fusion
Fact Sheet Cat. No. 110 402, 110 403
| Polyclonal rabbit antibody against syntaxin 1B | |
|---|---|
|
Cat. No. 110 402 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 110 403 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) 110 402
IP: yes ICC: 1 : 100 up to 1 : 1000 110 402 IHC: 1 : 1000 110 403 ELISA: yes (cat. no. 110 403; see remarks) |
| Immunogen | Synthetic peptide GKLAIFTDDIKMDSQMT (aa 171 - 187 in rat syntaxin 1B) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. |
| Reactivity | Human, rat, mouse, hamster, cow, chicken, zebrafish. |
| Specificity | Specific for syntaxin 1B, no cross reactivity to syntaxin 1A. |
| Remarks | ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 110 011 or 110 001 as capture antibodies (protocol for sandwich-ELISA). |
