Synapsins

Peripheral synaptic vesicle proteins; substrate for several protein kinases

Fact Sheet

Monoclonal mouse antibody against synapsin 1
Mouse Rabbit Guinea pig
Cat. No. 106 001 ascites	100 µl ascites, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Cat. No. 106 011C3 IgG, fluorescence-labeled	100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster^® 550. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex^® (Merck Chemicals) is not compatible with Oyster dyes!
Cat. No. 106 011C5 IgG, fluorescence-labeled	100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster^® 650. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex^® (Merck Chemicals) is not compatible with Oyster dyes!
Cat. No. 106 011BT IgG, biotin-labeled	100 µg purified IgG, lyophilized, biotin-labeled. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS.
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) 106 001 106 011BT IP: yes (see remarks) ICC: 1 : 500 up to 1 : 2000 106 001 106 011C3 106 011C5 IHC: 1 : 100 106 001 ELISA: yes (see remarks)
Clone	46.1
Subtype	IgG1
Immunogen	Electrophoretically purified rat synapsin 1.
Epitope	Binds to the proline rich D-domain of synapsin 1.
Reactivity	Human, rat, mouse, mammals; birds and lower vertebrates with lower reactivity.
Specificity	Specific for synapsin 1a and 1b independent of phosphorylation state.
Remarks	106 001:Protein A sepharose is recommended for immunoprecipitation, since this antibody does not bind efficiently to Protein G. Adjust pH to 8.6 to improve binding to protein A. 106 011BT: Avidin or streptavidin beads are recommended. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 106 002 as detector antibody (protocol for sandwich-ELISA).

Polyclonal rabbit antibody against synapsin 1,2
Cat. No. 106 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 500 image IHC: 1 : 500 image ELISA: yes (see remarks)
Immunogen	Synthetic peptide NYLRRRLSDSNFMANLPNGYMTDLRQP (aa 2 - 28 in rat synapsin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, hamster, cow, zebrafish image.
Specificity	Specific for synapsins 1a/b and 2a/b.
Remarks	ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 106 001 as capture antibody (protocol for sandwich-ELISA).

Polyclonal guinea pig antibody against synapsin 1,2
Cat. No. 106 004 crude antiserum	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: 1 : 1000 image IHC: 1 : 500 image
Immunogen	Synthetic peptide NYLRRRLSDSNFMANLPNGYMTDLRQP (aa 2 - 28 in rat synapsin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, hamster, cow.
Specificity	Specific for synapsins 1a/b and 2a/b.

Control peptide for the rabbit and guinea pig antibodies
Cat. No. 106-0P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (NYLRRRLSDSNFMANLPNGYMTDLRQP) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.