SV 2

Highly glycosylated synaptic vesicle protein with homology to transmembrane transporters

Fact Sheet

Polyclonal rabbit antibody against SV2 A
SV2 A SV2 B SV2 C
Cat. No. 119 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 200 image IHC: 1 : 500 image
Immunogen	Synthetic peptide EEGFRDRAAFIRGAKD (aa 2 - 17 in human) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, hamster, guinea pig, cow, chicken.
Specificity	Specific for SV2 A.
Remarks	SV2 A aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

Control peptide for SV2 A
Cat. No. 119-0P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (EEGFRDRAAFIRGAKD) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Polyclonal rabbit antibody against SV2 B
Cat. No. 119 102 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 200 image IHC: yes, works well with paraffin embedded tissues
Immunogen	Synthetic peptide DDYRYRDNYEGYAPND (aa 2 - 17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Rat, hamster, chicken; other species not tested yet.
Specificity	Specific for SV2 B.
Remarks	SV2 B aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

Control peptide for SV2 B
Cat. No. 119-1P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (DDYRYRDNYEGYAPND) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Polyclonal rabbit antibody against SV2 C
Cat. No. 119 202 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Cat. No. 119 203 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: 1 : 500 119 202 119 203 IHC: yes
Immunogen	Synthetic peptide of rat SV2 C (aa 2 - 16) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Reactivity	Human, rat, mouse, cow, dog.
Specificity	Specific for SV2 C.
Remarks	SV2 C aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

Control peptide for SV2 C
Cat. No. 119-2P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (aa 2-16 of rat SV2 C) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.