Snapin
Fact Sheet
| Polyclonal rabbit antibody 1, full length | |
|---|---|
|
Cat. No. 148 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 100 - 1 : 5000
IP: yes ICC: not tested yet IHC: 1 : 500 image |
| Immunogen | Recombinant full-length rat snapin. |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Specific for snapin. |
| Remarks | Since snapin is present in very low concentrations long exposure time is recommended. |
| Control protein for antiserum 1 | |
| Cat. No. 148-0P | 100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control peptide consists of the full-length rat snapin that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Polyclonal rabbit antibody 2, C-terminal peptide | |
|
Cat. No. 148 102 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 100 - 1 : 5000 (AP staining) blot
IP: not recommended ICC: not tested yet IHC: not recommended |
| Immunogen | Synthetic peptide TARRRAMLDSGVYPPGSPSK (aa 117 - 136 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Specific for snapin. |
| Remarks | Since snapin is present in very low concentrations long exposure time is recommended. |
| Control peptide for antiserum 2 | |
| Cat. No. 148-1P |
100 µg lyophilized peptide. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized! |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control peptide consists of the peptide (TARRRAMLDSGVYPPGSPSK) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
