SNAP 25

Monoclonal mouse antibody, clone 71.2; non-conserved epitope
Cat. No. 111 111 IgG, unlabeled	50 µg purified IgG, lyophilized. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in 20 mM TRIS, pH 7.7. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 10000 (AP staining) blot IP: yes (see remarks) ICC: 1 : 100 up to 1 : 500 image IHC: 1 : 1000 image ELISA: yes (see remarks)
Clone	71.2
Subtype	IgG1
Immunogen	Recombinant full length rat SNAP 25.
Epitope	Close to the N-terminus of the protein (aa 1 - 20).
Reactivity	Human, rat, mouse, vertebrates.
Specificity	Specific for vertebrate SNAP 25.
Remarks	Immunoprecipitation not quantitative, appears to depend on the binding status of the protein. Recognizes the Botulinum neurotoxin A and E cleavage products. Recognizes splice variants SNAP 25A and B. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 111 002 as detector antibody (protocol for sandwich-ELISA).