Synaptotagmin 7
A Ca2+ dependent regulator of exocytosis
Fact Sheet
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 105 172 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 105 173 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 100 up to 1 : 1000 (AP staining) 105 172 105 173
IP: not tested yet ICC: 1 : 200 (see remarks) 105 173 IHC: yes |
| Immunogen | Strep-Tag® fusion protein (aa 46 - 133) of rat synaptotagmin 7 containing the connecting sequence of the smallest nontruncated splice variant. |
| Reactivity | Human, rat, mouse, hamster. |
| Specificity | Recognizes synaptotagmin 7 (45 kDa) and splice variants C, D, E. |
| Remarks | ICC: Methanol fixation recommended. |
| Control protein | |
| Cat. No. 105-71P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the Strep-Tag® fusion protein (aa 46 - 133 of rat synaptotagmin 7) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
