Synaptotagmin 7
A Ca2+ dependent regulator of exocytosis
Fact Sheet Cat. No. 105 172, 105 173
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 105 172 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 105 173 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 100 up to 1 : 1000 (AP staining) 105 172 105 173
IP: not tested yet ICC: 1 : 200 (see remarks) 105 173 IHC: yes |
| Immunogen | Strep-Tag® fusion protein (aa 46 - 133) of rat synaptotagmin 7 containing the connecting sequence of the smallest nontruncated splice variant. |
| Reactivity | Human, rat, mouse, hamster. |
| Specificity | Recognizes synaptotagmin 7 (45 kDa) and splice variants C, D, E. |
| Remarks | ICC: Methanol fixation recommended. |
