Synaptotagmin 4
A protein targeted to the Golgi compartment and LDCVs in NGF differentiated PC12 cells
Fact Sheet
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 105 043 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 100 up to 1 : 1000 (AP staining) blot
IP: yes ICC: 1 : 500 image IHC: not tested yet |
| Immunogen | Recombinant rat synaptotagmin 4 (aa 40-151; spacer sequence responsible for Golgi targeting). |
| Reactivity | Rat and mouse; other species not tested yet. |
| Specificity | Specific for synaptotagmin 4. |
| Control protein | |
| Cat. No. 105-4P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the recombinant protein (aa 40-151 of rat synaptotagmin 4) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
