Synaptotagmin 2
An alternative Ca2+-sensor for neurotransmitter release
Fact Sheet
| Polyclonal rabbit antibody, cytoplasmic domain | |
|---|---|
|
Cat. No. 105 123 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: not tested yet IHC: 1 : 500 up to 1 : 1000 image |
| Immunogen | Synthetic peptide (aa 406-422 in rat synaptotagmin 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Specific for synaptotagmin 2. |
| Control peptide for cytoplasmic domain antibody | |
| Cat. No. 105-12P |
100 µg lyophilized peptide. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized! |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control peptide consists of the synthetic peptide (aa 406-422 in rat synaptotagmin 2) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
