Synaptotagmin 2
An alternative Ca2+-sensor for neurotransmitter release
Fact Sheet Cat. No. 105 223
| Polyclonal rabbit antibody, lumenal domain | |
|---|---|
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Cat. No. 105 223 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: 1 : 100 (see remarks) image IHC: not tested yet |
| Immunogen | Synthetic peptide (aa 1-11 in mouse synaptotagmin 2) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for synaptotagmin 2. |
| Remarks | This antibody can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals. |
