Synaptotagmin 1

Ca²⁺-sensor on synaptic vesicles, triggers neurotransmitter release

Fact Sheet

Monoclonal mouse antibody, cytoplasmic domain
cytoplasmic, mouse -, rabbit lumenal, mouse -, rabbit -, guinea pig
Cat. No. 105 011 IgG, unlabeled	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Cat. No. 105 011C3 IgG, Oyster^® 550-labeled	100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster^® 550. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex^® (Merck Chemicals) is not compatible with Oyster dyes!
Cat. No. 105 001 ascites	100 µl ascites, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining; see remarks) blot IP: yes ICC: 1 : 100 105 011C3 IHC: yes ELISA: yes (see remarks)
Clone	41.1
Subtype	IgG2a
Immunogen	Recombinant protein of rat synaptotagmin 1 (aa 80 - 421) containing the C-terminal part.
Epitope	Area around the N-terminal portion of the first C2-domain.
Reactivity	Human, rat, mouse, mammals.
Specificity	Specific for mammalian synaptotagmin 1, no cross-reactivity to other synaptotagmins.
Remarks	WB: Use only non-boiled samples when running SDS-PAGE. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 105 002 as detector antibody (protocol for sandwich-ELISA). Since synaptotagmin 1 is unevenly expressed in neuronal subpopulations and may, in fact, be missing from some, it is less well suited as a general marker for synapses.

Polyclonal rabbit antibody, cytoplasmic domain
Cat. No. 105 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 1000 image IHC: 1 : 500 (also paraffin embedded tissues) image ELISA: yes (see remarks)
Immunogen	Synthetic peptide DDDAETGLTDGE (aa 120 - 131 in rat synaptotagmin 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, cow, chicken, goldfish, zebrafish image.
Specificity	Some cross-reactivity to synaptotagmin 2.
Remarks	ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 105 011 or 105 001 as capture antibodies (protocol for sandwich-ELISA).

Control peptide for rabbit antibody, cytoplasmic domain
Cat. No. 105-0P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (DDDAETGLTDGE) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Monoclonal mouse antibody, lumenal domain, clone 604.4
Cat. No. 105 101 ascites	100 µl ascites, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 100 IHC: not tested yet
Clone	604.4
Subtype	IgG1
Immunogen	Synthetic peptide (aa 1 - 19 of rat synaptotagmin 1) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Reactivity	Rat, no signal in mouse.
Specificity	Specific for rat synaptotagmin 1, no cross-reactivity to other synaptotagmins.
Remarks	This antibody can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals. This antibody is of high affinity but not as strong as clone 41.1. In contrast to clone 604.1, it does not bind well to Protein G or Protein A.

Monoclonal mouse antibody, lumenal domain, clone 604.1
Cat. No. 105 221 hybridoma supernatant	200 µl hybridoma supernatant, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1: 10 000 (AP staining) IP: yes ICC: 1 : 100 image IHC: not tested yet
Clone	604.1
Subtype	IgG3
Immunogen	Synthetic peptide (aa 1 - 19 of rat synaptotagmin 1) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Reactivity	Rat, no signal in mouse.
Specificity	Specific for rat synaptotagmin 1, no cross-reactivity to other synaptotagmins.
Remarks	This antibody can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals. This antibody is of high affinity but not as strong as clone 41.1. This antibody is strongly recommended when binding to Protein G is required.

Monoclonal mouse antibody, lumenal domain, clone 604.2
Cat. No. 105 311 IgG, unlabeled	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Cat. No. 105 311C3 IgG, Oyster^® 550-labeled	100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster^® 550. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex^® (Merck Chemicals) is not compatible with Oyster dyes!
Cat. No. 105 311C5 IgG, Oyster^® 650-labeled	100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster^® 650. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex^® (Merck Chemicals) is not compatible with Oyster dyes!
Cat. No. 105 311AT1 IgG, ATTO^® 647N-labeled	100 µg purified IgG, lyophilized, fluorescence-labeled with ATTO^® 647N. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Cat. No. 105 311CpH IgG, CypHer-labeled	100 µg purified IgG, lyophilized, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Cat. No. 105 311BT IgG, biotin-labeled	100 µg purified IgG, lyophilized, biotin-labeled. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (105 311BT) IP: yes (105 311BT) ICC: 1 : 50 up to 1 : 300 105 311C3 105 311C5 105 311AT1 105 311CpH IHC: not tested yet
Clone	604.2
Subtype	IgG1
Immunogen	Synthetic peptide (aa 1 - 19 of rat synaptotagmin 1) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Reactivity	Rat, no signal in mouse.
Specificity	Specific for rat synaptotagmin 1, no cross-reactivity to other synaptotagmins.
Remarks	This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.

Control peptide for lumenal domain mouse antibodies
Cat. No. 105-1P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (aa 1 - 19 of rat synaptotagmin 1) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Polyclonal rabbit antibody, lumenal domain
Cat. No. 105 102 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Cat. No. 105 103C3 purified antibody, Oyster^® 550-labeled	50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Oyster^® 550. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex^® (Merck Chemicals) is not compatible with Oyster dyes!
Applications	WB: 1 : 1000 (AP staining) 105 102 IP: yes ICC: 1 : 100 105 102 105 103C3 IHC: 1 : 500 105 102
Immunogen	Synthetic peptide MVSASRPE (aa 1 - 8 in mouse synaptotagmin 1) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Reactivity	Rat, mouse; other species not tested yet
Specificity	Specific for synaptotagmin 1, no cross-reactivity to synaptotagmin 2.
Remarks	This antibody can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals.

Polyclonal guinea pig antibody, lumenal domain
Cat. No. 105 104 crude antiserum	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Cat. No. 105 105 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Guinea pig serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use.
Applications	WB: 1 : 2000 (AP staining) 105 104 105 105 IP: not tested yet ICC: 1 : 500 up to 1 : 5000 105 104 105 105 IHC: not tested yet
Immunogen	Synthetic peptide MVSASRPE (aa 1 - 8 in mouse synaptotagmin 1) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Reactivity	Rat, mouse; other species not tested yet
Specificity	Specific for synaptotagmin 1, no cross-reactivity to synaptotagmin 2.
Remarks	This antibody can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals.

Synaptotagmin 1

Ca2+-sensor on synaptic vesicles, triggers neurotransmitter release

Fact Sheet

Ca²⁺-sensor on synaptic vesicles, triggers neurotransmitter release