Synaptotagmin 1
Ca2+-sensor on synaptic vesicles, triggers neurotransmitter release
Fact Sheet Cat. No. 105 311, 105 311C2, -C3, -C5, -AT1, -CR1, -CpH, -BT
| Monoclonal mouse antibody, clone 604.2; lumenal domain | |
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Cat. No. 105 311 IgG, unlabeled |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
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Cat. No. 105 311C2 IgG, Oyster® 488-labeled |
100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster® 488. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex® (Merck Chemicals) is not compatible with Oyster dyes! |
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Cat. No. 105 311C3 IgG, Oyster® 550-labeled |
100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster® 550. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex® (Merck Chemicals) is not compatible with Oyster dyes! |
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Cat. No. 105 311C5 IgG, Oyster® 650-labeled |
100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster® 650. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex® (Merck Chemicals) is not compatible with Oyster dyes! |
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Cat. No. 105 311AT1 IgG, ATTO® 647N-labeled |
100 µg purified IgG, lyophilized, fluorescence-labeled with ATTO® 647N. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
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Cat. No. 105 311CR1 IgG, Chromeo® 488-labeled |
100 µg purified IgG, lyophilized, fluorescence-labeled with Chromeo®488. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
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Cat. No. 105 311CpH IgG, CypHer-labeled |
100 µg purified IgG, lyophilized, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
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Cat. No. 105 311BT IgG, biotin-labeled |
100 µg purified IgG, lyophilized, biotin-labeled. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 105 311
IP: yes ICC: 1 : 50 up to 1 : 300 105 311C2 105 311C3 105 311C5 105 311AT1 105 311CR1 105 311CpH IHC: not tested yet |
| Clone | 604.2 |
| Subtype | IgG1 |
| Immunogen | Synthetic peptide (aa 1 - 19 of rat synaptotagmin 1) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue. |
| Reactivity | Rat, no signal in mouse. |
| Specificity | Specific for rat synaptotagmin 1, no cross-reactivity to other synaptotagmins. |
| Remarks | This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures. |
