Synaptoporin (Synaptophysin 2)
Synaptic vesicle membrane protein similar to synaptophysin 1
Fact Sheet
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 102 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: yes ICC: 1 : 100 up to 1 : 1000 image IHC: 1 : 500 image |
| Immunogen | Synthetic peptide EFGQQPSGPTSFNN (aa 250-263 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. |
| Reactivity | Rat, mouse hamster; other species not tested yet. |
| Specificity | Specific for synaptoporin. |
| Control peptide | |
| Cat. No. 102-0P |
100 µg lyophilized peptide. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized! |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control peptide consists of the synthetic peptide (EFGQQPSGPTSFNN) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
