Synaptopodin
An actin associated protein present in renal podocytes and telencephalic dendrites
Fact Sheet
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 163 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: 1 : 600 image IHC: 1 : 250 up to 1 : 500 image |
| Immunogen | Recombinant mouse synaptopodin (aa 331 - 452 in isoform 2). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Fragment used for immunization is present in all 3 isoforms. |
| Polyclonal guinea pig antibody | |
|
Cat. No. 163 004 crude antiserum |
100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 500 (AP staining; see remarks)
IP: not tested yet ICC: 1 : 500 image IHC: 1 : 200 image |
| Immunogen | Recombinant mouse synaptopodin (aa 331 - 452 in isoform 2). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Fragment used for immunization is present in all 3 isoforms. |
| Remarks | WB: Application is limited, the antibody is less sensitive than the rabbit antibody. |
| Control protein | |
| Cat. No. 163-0P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the recombinant protein (aa 331 - 452) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
