Synaptophysin 1

Major synaptic vesicle membrane protein; neuroendocrine tumor marker

Fact Sheet

Monoclonal mouse antibody
mouse - rabbit - guinea pig antibodies control peptide
Cat. No. 101 011 IgG, unlabeled	50 µg purified IgG, lyophilized. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Cat. No. 101 011C3 IgG, fluorescence-labeled	50 µg purified IgG, lyophilized, fluorescence-labeled with Oyster^® 550. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex^® (Merck Chemicals) is not compatible with Oyster dyes!
Cat. No. 101 011C5 IgG, fluorescence-labeled	50 µg purified IgG, lyophilized, fluorescence-labeled with Oyster^® 650. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex^® (Merck Chemicals) is not compatible with Oyster dyes!
Cat. No. 101 011BT IgG, biotin-labeled	50 µg purified IgG, lyophilized, biotin-labeled. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 10000 (AP staining) 101 011 101 011BT IP: yes ICC: 1 : 100 up to 1 : 1000 101 011C5 101 011BT IHC: 1 : 500 up to 1 : 1000 101 011 101 011C3 101 011C5 ELISA: yes (see remarks)
Clone	7.2
Subtype	IgG1
Immunogen	Electrophoretically purified synaptophysin.
Epitope	Reacts with cytoplasmic tail.
Reactivity	Human, rat, mouse, mammals; weaker reaction in birds, reptilia, amphibia, zebrafish.
Specificity	Specific for synaptophysin 1, no cross-reactivity to other synaptophysins.
Remarks	Widely used as marker for nerve terminals and neuroendocrine tumors. Works well with paraffin embedded material. For still unknown reason, neuronal synaptophysin is better recognised than neuroendocrine synaptophysin. If this is a problem, the polyclonal rabbit antibody, cat. no. 101 002, is recommended. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 101 002 as detector antibody (protocol for sandwich-ELISA).

Polyclonal rabbit antibody
Cat. No. 101 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) blot IP: yes ICC: 1 : 100 up to 1 : 500 image IHC: yes image ELISA: yes (see remarks)
Immunogen	Synthetic peptide GPQGAPTSFSNQM (aa 301 - 313 in human synaptophysin 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, hamster, cow, chicken, frog, zebrafish.
Specificity	Specific for synaptophysin 1, no cross-reactivity to other synaptophysins.
Remarks	ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 101 011 as capture antibody (protocol for sandwich-ELISA).

Polyclonal guinea pig antibody
Cat. No. 101 004 crude antiserum	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 1000 image IHC: 1 : 500 image
Immunogen	Synthetic peptide GPQGAPTSFSNQM (aa 301 - 313 in human synaptophysin 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, hamster, cow, chicken, frog.
Specificity	Specific for synaptophysin 1, no cross-reactivity to other synaptophysins.

Control peptide for rabbit and guinea pig antibodies
Cat. No. 101-0P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (GPQGAPTSFSNQM) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.