| Polyclonal rabbit antibody 2; splice variant | |
|---|---|
|
Cat. No. 145 103 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 200 up to 1 : 2000 (AP staining) blot
IP: not tested yet ICC: not tested yet IHC: 1 : 1000 up to 1 : 5000 image |
| Immunogen | Synthetic peptide CGVGAPPSPGVTRREME (aa 1140 - 1155 in isoform 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. |
| Reactivity | Rat, mouse, hamster; other species not tested yet. |
| Specificity | Recognizes isoforms 1, 2, 3 of synaptojanin 1. |
| Remarks | Running SDS-PAGE with non-boiled samples gives stronger signals. |