Synaptogyrin 1

Major synaptic vesicle membrane protein; tyrosine-phosphorylated

Fact Sheet

Monoclonal mouse antibody
Cat. No. 103 011 IgG, unlabeled	50 µg purified IgG, lyophilized. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes (see remarks) ICC: 1 : 100 IHC: yes ELISA: yes (see remarks)
Clone	80.1
Subtype	IgG1
Immunogen	Recombinant rat synaptogyrin 1 (aa 170 - 234).
Reactivity	Human, rat, mouse, hamster, no signal in chicken.
Specificity	Specific for synaptogyrin 1 with a weak side-reactivity towards tubulin.
Remarks	IP: In immunoprecipitation (but not in other assays) tubulin coprecipitates with synaptogyrin 1 in about equal proportions. This is due to a direct binding of the antibody to tubulin. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 103 002 as detector antibody (protocol for sandwich-ELISA).

Polyclonal rabbit antibody
Cat. No. 103 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) blot IP: not tested yet ICC: 1 : 100 up to 1 : 500 IHC: not tested yet ELISA: yes (see remarks)
Immunogen	Synthetic peptide NAFDAEPQGYQSQGY (aa 220 - 234 in rat synaptogyrin 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, hamster, chicken.
Specificity	Specific for synaptogyrin 1.
Remarks	ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 103 011 as capture antibody (protocol for sandwich-ELISA).

Control peptide for the rabbit antibody
Cat. No. 103-0P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (NAFDAEPQGYQSQGY) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.