RIM 1
A putative Rab 3 effector protein of the active zone
Fact Sheet
| Polyclonal rabbit antibody 1 against RIM 1 | |
|---|---|
|
Cat. No. 140 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 100 up to 1 : 1000 (AP staining) blot
IP: not tested yet ICC: 1 : 200 up to 1 : 500 image IHC: yes, paraformaldehyde and methanol fixation |
| Immunogen | Recombinant protein (aa 596 - 705) of rat RIM 1 containing part of PDZ domain. |
| Reactivity | Rat, mouse, hamster, chicken, frog. |
| Specificity | Specific for RIM 1 with weak cross reactivity to RIM 2. |
| Polyclonal rabbit antibody 2 against RIM 1 | |
|
Cat. No. 140 013 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: not recommended (see remarks)
IP: not tested yet ICC: 1 : 500 up to 1 : 1000 image IHC: 1 : 500 image |
| Immunogen | Recombinant protein (aa 207 - 366) of rat RIM 1, a stretch of divergent aa connecting the Rab binding motif and the PDZ domain. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for RIM 1, no cross reactivity to RIM 2. |
| Remarks | WB: Antibody 3 is recommended. |
| Polyclonal rabbit antibody 3 against RIM 1 | |
|
Cat. No. 140 023 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining; see remarks) blot
IP: not tested yet ICC: not tested yet IHC: not tested yet |
| Immunogen | Recombinant protein (aa 945 - 1185) of rat RIM 1, a stretch of divergent aa connecting the C1 and C2 domain. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for RIM 1, no cross reactivity to RIM 2. |
| Remarks | WB: This antibody is recommended. |
| Polyclonal rabbit antibody against RIM 1/2, Zn-finger domain | |
|
Cat. No. 140 203 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: yes ICC: 1 : 500 image IHC: 1 : 500 (see remarks) image |
| Immunogen | Recombinant protein (aa 1 - 466) of rat RIM 2 containing the Zn-finger domain. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | RIM 2 including splice variants, cross reacts to RIM 1. |
| Remarks |
IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008). Cell-type-dependent molecular composition of the axon initial segment. Journal of Neuroscience 28: 14329-40. |
