RIM 1
A putative Rab 3 effector protein of the active zone
Fact Sheet Cat. No. 140 203
| Polyclonal rabbit antibody against RIM 1/2, Zn-finger domain | |
|---|---|
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Cat. No. 140 203 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: yes ICC: 1 : 500 image IHC: 1 : 500 (see remarks) image |
| Immunogen | Recombinant protein (aa 1 - 466) of rat RIM 2 containing the Zn-finger domain. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | RIM 2 including splice variants, cross reacts to RIM 1. |
| Remarks |
IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008). Cell-type-dependent molecular composition of the axon initial segment. Journal of Neuroscience 28: 14329-40. |
