Rabphilin 3

Polyclonal rabbit antibody
Cat. No. 118 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Cat. No. 118 003 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in Tris buffer. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) 118 002 118 003 IP: no ICC: yes IHC: not tested yet
Immunogen	Synthetic peptide WHQLQNENHVSSD (aa 671 - 684 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, hamster, cow.
Specificity	Specific for rabphilin 3a.

Control peptide
Cat. No. 118-0P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (WHQLQNENHVSSD) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.