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Rab 5

GTP-binding protein involved in endocytosis

Fact Sheet Cat. No. 108 101

Monoclonal mouse antibody, clone 621.4
Cat. No. 108 101
ascites
100 µl ascites, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 10000 (AP staining)
IP: yes
ICC: 1 : 100 up to 1 : 1000 (but see remarks)
IHC: not tested yet
Clone 621.4
Subtype IgG2a
Immunogen Recombinant full length rat rab 5a.
Reactivity Human, rat, mouse; other species not tested.
Specificity Specific for rab 5 (probably only rab 5a). No cross-reactivity to other rab proteins.
Remarks
  • ICC: Not suitable for ICC of aldehyde- fixed tissues or cells.
  • This antibody was used very successfully for immunoisolation of early endosomes and for the differentiation of early endosomes from related trafficking organelles in neurons and nonneuronal cells.


Synaptic Systems Gesellschaft für neurobiologische Forschung, Entwicklung und Produktion mbH | Rudolf-Wissell-Str. 28 | 37079 Goettingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com | Imprint
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com