Rab 3

Major rab protein in brain; highly enriched on synaptic vesicles

Fact Sheet

Monoclonal mouse antibody against Rab 3
Rab 3 Rab 3a Rab 3c Rab 3d
Cat. No. 107 011 IgG, unlabeled	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not recommended ICC: 1 : 100 up to 1 : 1000 image IHC: not tested yet
Clone	42.1
Subtype	IgG1
Immunogen	Recombinant full length rat Rab 3a.
Epitope	Localized to a stretch of amino acids in the middle of the molecule (aa 95 - 151).
Reactivity	Human, rat, mouse, vertebrates, some invertebrates.
Specificity	Specific for all four Rab 3 isoforms a, b, c d.

Polyclonal rabbit antibody against Rab 3
Cat. No. 107 003 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: 1 : 1000 image IHC: 1 : 200 up to 1 : 500 image
Immunogen	Recombinant human Rab 3c (aa 154 - 227).
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for all four Rab 3 isoforms a, b, c, d.
Remarks	This antibody has been affinity purified with the complete antigen (aa 154 - 227) and recognizes all isoforms, due to the homology of Rab 3 proteins.

Monoclonal mouse antibody against Rab 3a
Cat. No. 107 111 IgG, unlabeled	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 1000 up to 1 : 2000 image IHC: yes ELISA: yes (see remarks)
Clone	42.2
Subtype	IgG2b
Immunogen	Recombinant full length rat Rab 3a.
Epitope	Localized to a stretch of 20 amino acids in the C-terminal part of the molecule (aa 191 - 220.
Reactivity	Human, rat, mouse, mammals.
Specificity	Specific for mammalian Rab 3a.
Remarks	ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 107 102 as detector antibody (protocol for sandwich-ELISA).

Polyclonal rabbit antibody against Rab 3a
Cat. No. 107 102 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 1000 image IHC: yes image ELISA: yes (see remarks)
Immunogen	Synthetic peptide ASATDSRYGQKES (aa 2 - 14 in rat Rab 3a) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, hamster, cow, zebrafish.
Specificity	Specific for Rab 3a.
Remarks	ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 107 111 as capture antibody (protocol for sandwich-ELISA).

Control peptide for Rab 3a rabbit antibody
Cat. No. 107-1P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (ASATDSRYGQKES) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Polyclonal rabbit antibody against Rab 3c
Cat. No. 107 203 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 500 image IHC: not tested yet
Immunogen	Recombinant protein of human Rab 3c (aa 154 - 227).
Reactivity	Human, rat, mouse, cow, monkey.
Specificity	Specific for Rab 3c.
Remarks	This antibody has been affinity purified with a peptide consisting only of the Rab 3c specific epitope NTRLKETPPPPQPN (aa 211 - 224) of the human protein.

Polyclonal rabbit antibody against Rab 3d
Cat. No. 107 303 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: not tested yet IHC: not tested yet
Immunogen	A mixture of two peptides corresponding to aa 3-16 and aa 193-210 of rat Rab 3d, coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for Rab 3d, no cross reactivity to other Rab 3 proteins.
Remarks	This antibody has been purified over a full-length Rab 3d column.