Rab 27
A member of the Rab family responsible for melanosomal transport and hormone secretion
Fact Sheet
 Rab 27
Rab 27A
Rab 27B |
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Polyclonal rabbit antibody 1, against Rab 27 |
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Cat. No. 168 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: yes ICC: not tested yet IHC: not tested yet |
| Immunogen | Purified recombinant full length rat rab 27A. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Recognizes both isoforms rab 27A and B; no cross reaction to other rab proteins. |
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Polyclonal rabbit antibody 2, against Rab 27A |
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Cat. No. 168 013 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: yes ICC: not tested yet IHC: not tested yet |
| Immunogen | Purified recombinant full length rat rab 27A. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for rab 27A, no cross reaction to rab 27B. |
| Remarks | Only the non-conserved C-terminal part of the protein was used for affinity purification to achieve specificity. |
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Control protein for antibodies 1 and 2 |
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| Cat. No. 168-0P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the recombinant protein that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
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Polyclonal rabbit antibody 3, against Rab 27B |
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Cat. No. 168 103 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: yes ICC: 1 : 500 image IHC: not tested yet |
| Immunogen | Purified recombinant full length rat rab 27B. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for rab 27B with weak cross reaction to rab 27A. |
|
Control protein for antibody 3 |
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| Cat. No. 168-1P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the recombinant protein that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
