Pyro-Glu Abeta

A major component of neuritic plaques in Alzheimer's disease

Fact Sheet Cat. No. 218 511

Monoclonal mouse antibody against oligo-Abeta-pE3, clone 9D5
Cat. No. 218 511 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 500 (see remarks) blot IP: not tested yet ICC: not tested yet IHC: 1 : 100 image ELISA: yes (see remarks)
Clone	9D5
Subtype	IgG2b
Immunogen	oligomeric pyro-Glu Abeta 3-38
Reactivity	Human, mouse; other species not tested yet.
Specificity	Recognizes specific oligomeric structures formed preferentially by Abeta-pE3.
Remarks	WB: We recommend the Invitrogen NativePAGE system in combination with PVDF blotting membranes. Peptide preparation: Synthetic Abeta peptides were monomerized in 70 % formic acid, and the solvent was evaporated in a speed-vac immediately. Prior to each experiment, peptides were dissolved in 0.3 % ammonia, underwent ultrasonic treatment, and were further diluted to an end concentration of 0.15 % ammonia. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 218 011BT as detector antibody (protocol for sandwich-ELISA).
Important note for users	The mouse monoclonal antibody clone 9D5, Cat. No. 218 511, is patented (patent application PCT/EP2011/002739). By purchasing this antibody the customer acquires rights to use this product for research purposes only. Any diagnostic and therapeutic in vitro or in vivo use is explicitly excluded.