| Polyclonal guinea pig antibody | |
|---|---|
|
Cat. No. 142 104 crude antiserum |
100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: 1 : 500 up to 1 : 1000 image IHC: 1 : 200 image |
| Immunogen | Recombinant rat piccolo (aa 2011 - 2350). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for piccolo. |
| Remarks |
WB: Due to its large size, piccolo requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed.
This antibody detects an additional band of ~65 kDa. |