Parvalbumin

A calcium binding protein

Fact Sheet

Polyclonal rabbit antibody
Cat. No. 195 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Cat. No. 195 003BT biotin-labeled	100 µg protein G purified antibody, lyophilized, biotin-labeled. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 5000 up to 1 : 10000 (AP staining; see remarks) 195 002 IP: yes (cat. no. 195 002) ICC: not tested yet IHC: 1 : 100 up to 1 : 500 195 002 195 003BT
Immunogen	Recombinant full length rat parvalbumin.
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for parvalbumin.
Remarks	WB: Due to its small size a tricine gel is recommended.

Polyclonal guinea pig antibody
Cat. No. 195 004 crude antiserum	100 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining; see remarks) blot IP: not tested yet ICC: not tested yet IHC: 1 : 200 up to 1 : 500 image
Immunogen	Recombinant full length rat parvalbumin.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for parvalbumin.
Remarks	Due to its small size a tricine gel is recommended.

Control protein
Cat. No. 195-0P	100 µg lyophilized protein. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use.
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control protein consists of the full length rat parvalbumin that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

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