P 31 (Use 1)
A SNARE protein involved in retrograde Golgi to ER transport
Fact Sheet
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 187 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 5000 (AP staining) blot
IP: not tested yet ICC: yes IHC: not tested yet |
| Immunogen | Recombinant human p 31 (aa 4 - 232). |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Specific for p 31. |
| Control protein | |
| Cat. No. 187-0P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the recombinant protein (aa 4 - 232 of human p 31) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
