Proton ATPase (116 kDa subunit)
Largest subunit of the vacuolar proton pump in synaptic vesicles and coated vesicles
Fact Sheet Cat. No. 109 002, 109 003
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 109 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 109 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) 109 002 109 003
IP: not tested yet ICC: yes (only serum tested) IHC: 1 : 100 109 002 |
| Immunogen | Synthetic peptide FSFEHIREGKFDE (826-838 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. |
| Reactivity | Rat, mouse, hamster. |
| Specificity | Specific for the α1 116kDa subunit. |
| Remarks | The proton pump aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples. |
