NSF

Trimeric ATPase required for membrane fusion during vesicular transport

Fact Sheet

Monoclonal mouse antibody
Cat. No. 123 001 ascites	100 µl ascites, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 500 image IHC: 1 : 1000 image ELISA: yes (see remarks)
Clone	83.11
Subtype	IgG1
Immunogen	Recombinant full length rat NSF.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for NSF.
Remarks	ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 123 002 as detector antibody (protocol for sandwich-ELISA).

Polyclonal rabbit antibody
Cat. No. 123 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 100 up to 1 : 1000 image IHC: yes (paraffin sections) ELISA: yes (see remarks)
Immunogen	Synthetic peptide REEGASPLDFD (aa 733 - 744 in rat NSF) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, chinese hamster.
Specificity	Specific for NSF.
Remarks	ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 123 001 as capture antibody (protocol for sandwich-ELISA).

Control peptide for the rabbit antibody
Cat. No. 123-0P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (REEGASPLDFD) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.