NP-EGTA
A photolabile Ca2+ chelator
General references NP-EGTA
Sorensen JB, Fernandez-Chacon R, Sudhof TC & Neher E (2003). Examining synaptotagmin 1 function in dense core vesicle exocytosis under direct control of Ca2+. Journal of General Physiology 122: 265-76.Nagy G, Matti U, Nehring RB, Binz T, Rettig J, Neher E & Sorensen JB (2002). Protein kinase C-dependent phosphorylation of synaptosome-associated protein of 25 kDa at Ser187 potentiates vesicle recruitment. Journal of Neuroscience 22: 9278-86.
Ashery U, Varoqueaux F, Voets T, Betz A, Thakur P, Koch H, Neher E, Brose N & Rettig J (2000). Munc13-1 acts as a priming factor for large dense-core vesicles in bovine chromaffin cells. EMBO Journal 19: 3586-96.
Wei S, Xu T, Ashery U, Kollewe A, Matti U, Antonin W, Rettig J & Neher E (2000). Exocytotic mechanism studied by truncated and zero layer mutants of the C-terminus of SNAP-25. EMBO Journal 19: 1279-89.
Xu T, Ashery U, Burgoyne RD & Neher E (1999). Early requirement for alpha-SNAP and NSF in the secretory cascade in chromaffin cells. EMBO Journal 18: 3293-304.
Fleet A, Ellis-Davies G & Bolsover S (1998). Calcium buffering capacity of neuronal cell cytosol measured by flash photolysis of calcium buffer NP-EGTA. Biochemical and Biophysical Research Communications 250: 786-90.
Parsons TD, Ellis-Davies GC & Almers W (1996). Millisecond studies of calcium-dependent exocytosis in pituitary melanotrophs: comparison of the photolabile calcium chelators nitrophenyl-EGTA and DM-nitrophen. Cell Calcium 19: 185-92.
Ellis-Davies GC, Kaplan JH & Barsotti RJ (1996). Laser photolysis of caged calcium: rates of calcium release by nitrophenyl-EGTA and DM-nitrophen. Biophysical Journal 70: 1006-16.
Zimmermann B, Somlyo AV, Ellis-Davies GC, Kaplan JH & Somlyo AP (1995). Kinetics of prephosphorylation reactions and myosin light chain phosphorylation in smooth muscle. Flash photolysis studies with caged calcium and caged ATP. Journal of Biological Chemistry 270: 23966-74.
Ellis-Davies GC & Kaplan JH (1994). Nitrophenyl-EGTA, a photolabile chelator that selectively binds Ca2+ with high affinity and releases it rapidly upon photolysis. Proceedings of the National Academy of Sciences USA 91: 187-91.
