GluN 1 (NMDA-Receptor 1)
Major subunit of glutamate receptor involved in synaptic plasticity
Fact Sheet Cat. No. 114 103
| Polyclonal rabbit antibody 2 | |
|---|---|
|
Cat. No. 114 103 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: not recommended (see remarks)
IP: not tested yet ICC: not recommended IHC: 1 : 500 up to 1 : 1000 (see remarks) image |
| Immunogen | Synthetic peptide (aa 35-53 of rat GluN 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Specific for GluN 1. |
| Remarks |
WB: Antibody 1, cat. no. 114 003, is recommended for WB.
IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008). Cell-type-dependent molecular composition of the axon initial segment. Journal of Neuroscience 28: 14329-40. |
