Neuroligin 2
A postsynaptic cell adhesion molecule localized to postsynaptic densities of excitatory synapses
Fact Sheet
| Monoclonal mouse antibody against neuroligin 1,2,3; clone 4F9 | |
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Cat. No. 129 011 IgG, unlabeled |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: not recommended IHC: not tested yet ELISA: yes (see remarks) |
| Clone | 4F9 |
| Subtype | IgG2a |
| Immunogen | Recombinant protein of rat neuroligin 1 containing the extracellular sequence (aa 1 - 695). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Recognizes rat and mouse neuroligins 1, 2, 3, and probably also neuroligin 4. |
| Remarks | ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 129 203 as detector antibody (protocol for sandwich-ELISA). |
| Monoclonal mouse antibody against neuroligin1,2,3,4; clone 87H9 | |
|
Cat. No. 129 211 IgG, unlabeled |
100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: 1 : 200 up to 1 : 500 image IHC: not tested yet |
| Clone | 87H9 |
| Subtype | IgG1 |
| Immunogen | Recombinant protein of mouse neuroligin 3 (cyotplasmic tail, aa 608 - 722). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity |
WB: Recognizes neuroligin 2 and 3. ICC: Stains predominantly neuroligin 2 in cultured hippocampus neurons. Detects transiently expressed GFP fusion proteins comprising the cytoplasmic tails of neuroligins 1-4. |
| Polyclonal rabbit antibody against neuroligin 2 | |
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Cat. No. 129 202 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 129 203 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining; see remarks) 129 202
IP: not tested yet ICC: yes (see remarks!) 129 203 IHC: 129 203: yes image 129 202: not tested yet ELISA: yes (cat. no. 129 203; see remarks) |
| Immunogen |
129 202: Mixture of synthetic peptides of rat neuroligin 2 (aa 732-749 and aa 750-767) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue. 129 203: Synthetic peptide of rat neuroligin 2 (aa 750-767) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue. |
| Reactivity | Human, rat, mouse, monkey, ape, cow. |
| Specificity | Specific for neuroligin 2, no cross reactivity to neuroligins 1, 3, 4. |
| Remarks |
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