Neuroligin 1
A postsynaptic cell adhesion molecule localized to postsynaptic densities of excitatory synapses
Fact Sheet
| Monoclonal mouse antibody against neuroligin 1,2,3; clone 4F9 | |
|---|---|
|
Cat. No. 129 011 IgG, unlabeled |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: not recommended IHC: not tested yet ELISA: yes |
| Clone | 4F9 |
| Subtype | IgG2a |
| Immunogen | Recombinant protein of rat neuroligin 1 containing the extracellular sequence (aa 1 - 695). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Recognizes rat and mouse neuroligins 1, 2, 3, and probably also neuroligin 4. |
| Monoclonal mouse antibody against neuroligin 1,2,3,4; clone 87H9 | |
|
Cat. No. 129 211 IgG, unlabeled |
100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: 1 : 200 up to 1 : 500 image IHC: not tested yet |
| Clone | 87H9 |
| Subtype | IgG1 |
| Immunogen | Recombinant protein of mouse neuroligin 3 (cyotplasmic tail, aa 608 - 722). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity |
WB: Recognizes neuroligin 2 and 3. ICC: Stains predominantly neuroligin 2 in cultured hippocampus neurons. Detects transiently expressed GFP fusion proteins comprising the cytoplasmic tails of neuroligins 1-4. |
| Monoclonal mouse antibody against neuroligin 1; clone 4C12 | |
|
Cat. No. 129 111 IgG, unlabeled |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: not recommended IHC: not recommended |
| Clone | 4C12 |
| Subtype | IgG1 |
| Immunogen | Recombinant protein of rat neuroligin 1 containing the extracellular sequence (aa 1 - 695). |
| Epitope | Binds within aa 46 - 165 of rat neuroligin 1 isoform 1 (unprocessed precursor). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Epitope present in all 4 isoforms of neuroligin 1; no cross reactivity to neuroligins 2, 3, 4. |
| Polyclonal rabbit antibody 1 against neuroligin 1, extracellular domain | |
|
Cat. No. 129 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: not recommended IHC: not recommended |
| Immunogen | Recombinant protein of rat neuroligin 1 containing the extracellular sequence (aa 46 - 165). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for neuroligin 1, no cross reactivity to neuroligins 2, 3, 4. |
| Control protein for neuroligin 1 mouse, clone 4C12, and rabbit antibody 1 | |
| Cat. No. 129-0P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of aa 46 - 165 of rat neuroligin 1. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Polyclonal rabbit antibody 2 against neuroligin 1, cytoplasmic domain | |
|
Cat. No. 129 013 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: not tested yet IHC: not tested yet |
| Immunogen | Purified recombinant protein of rat neuroligin 1 (cytoplasmic domain; aa 718 - 843 from the unprocessed precursor). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for neuroligin 1, no cross reactivity to neuroligins 2, 3, 4. |
