Munc 18 (rb-sec1, n-sec1; stxbp2)
Peripheral membrane protein involved in vesicle docking and fusion
Fact Sheet
 Munc 18-1 antibodies
Munc 18-2 antibodies |
|
 Monoclonal mouse antibody against Munc 18-1 |
|
|---|---|
|
Cat. No. 116 011 IgG, unlabeled |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 5000 (AP staining) blot
IP: not tested yet ICC: 1 : 1000 image IHC: not tested yet ELISA: yes (see remarks) |
| Clone | 131.1 |
| Subtype | IgG2a |
| Immunogen | Full length recombinant rat munc 18-1. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for munc 18-1 with a minor cross-reactivity to munc 18-2. |
| Remarks |
- This antibody detects two smaller bands (possible degradation products) of unkown identity. - ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 116 002 as detector antibody (protocol for sandwich-ELISA). |
| Polyclonal rabbit antibody against Munc 18-1 |
|
|
Cat. No. 116 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: yes ICC: 1 : 500 up to 1 : 1000 IHC: yes ELISA: yes (see remarks) |
| Immunogen | Synthetic peptide DTLKKLNKTDEEISS (aa 580 - 594 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. |
| Reactivity | Human, rat, mouse, hamster, cow. |
| Specificity | Specific for Munc 18-1. |
| Remarks | ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 116 011 as capture antibody (protocol for sandwich-ELISA). |
|
Control peptide for munc 18-1 rabbit antibody |
|
| Cat. No. 116-0P |
100 µg lyophilized peptide. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized! |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control peptide consists of the synthetic peptide (DTLKKLNKTDEEISS) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Polyclonal rabbit antibody against Munc 18-2 |
|
|
Cat. No. 116 102 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 5000 (AP staining) blot
IP: not tested yet ICC: 1 : 500 IHC: not tested yet |
| Immunogen | Full length recombinant mouse Munc18-2. |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for Munc 18-2 with weak cross-reactivity to Munc18-1 and 3. |
| Remarks | This antibody detects two smaller bands (possible degradation products) of unkown identity. |
