Munc 18 (rb-sec1, n-sec1)

Peripheral membrane protein involved in vesicle docking and fusion

Fact Sheet

Monoclonal mouse antibody
Cat. No. 116 011 IgG, unlabeled	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) blot IP: not tested yet ICC: 1 : 1000 image IHC: yes
Clone	131.1
Subtype	IgG2a
Immunogen	Full length recombinant rat munc 18-1.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for munc 18-1 with a minor cross-reactivity to munc 18-2.
Remarks	This antibody detects two smaller bands (possible degradation products) of unkown identity.

Polyclonal rabbit antibody
Cat. No. 116 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 500 up to 1 : 1000 IHC: not tested yet
Immunogen	Synthetic peptide DTLKKLNKTDEEISS (aa 580 - 594 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity	Human, rat, mouse, hamster, cow.
Specificity	Specific for Munc 18-1.

Control peptide for rabbit antibody
Cat. No. 116-0P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (DTLKKLNKTDEEISS) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.