Munc 13

Mammalian homologues of the C. elegans unc-13 gene essential for exocytosis

Fact Sheet

Monoclonal mouse antibody
Cat. No. 126 111 IgG, unlabeled	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 500 up to 1 : 5000 (AP staining) blot IP: not tested yet ICC: limited (see remarks) IHC: not tested yet ELISA: yes (see remarks)
Clone	266B1
Subtype	Ig2b
Immunogen	Recombinant rat munc 13-1 (aa 3 - 317).
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for munc 13-1.
Remarks	ICC: limited to methanol fixation. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 126 103 as detector antibody (protocol for sandwich-ELISA).

Polyclonal rabbit antibody
Cat. No. 126 102 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Cat. No. 126 103 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) 126 102 126 103 IP: not tested yet ICC: yes (see remarks) 126 103 IHC: yes ELISA: yes (cat. no. 126 103; see remarks)
Immunogen	Recombinant rat munc 13-1 (aa 3 - 317).
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for munc 13-1.
Remarks	- This antibody gives much better results in ICC than the monoclonal antibody. - ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 126 111 as capture antibody (protocol for sandwich-ELISA).