MLC-2V

A myosin specific for the ventricle of the mammalian heart

Fact Sheet

Monoclonal mouse antibody, clone 330G5
Cat. No. 310 111 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Cat. No. 310 111AT1 purified antibody, ATTO^® 647N-labeled	100 µg purified IgG, lyophilized, fluorescence-labeled with ATTO^® 647N. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Cat. No. 310 111AT2 purified antibody, ATTO^® 550-labeled	100 µg purified IgG, lyophilized, fluorescence-labeled with ATTO^® 550. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 10000 (ECL) 310 111 IP: yes ICC: yes (methanol fixation) 310 111 IHC: 1 : 200 310 111 310 111AT1 310 111AT2
Clone	330G5
Subtype	IgG2a
Immunogen	Recombinant protein of human myosin light chain 2V.
Epitope	FDPEGKG
Reactivity	Human, rat, mouse, pig, chicken; other species not tested yet.
Specificity	Specific for MLC-2V, no cross-reactivity to MLC-2A.
Remarks	Protocol for immunohistochemistry of this antibody will be provided with the goods.

Polyclonal rabbit antibody 1
Cat. No. 310 003 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: not tested yet IHC: 1 : 200 image
Immunogen	Synthetic peptide (aa 104-118 of mouse MLC-2V) coupled to KLH via an N-terminal added cysteine.
Reactivity	Human, rat, mouse, pig, chicken; other species not tested yet.
Specificity	Specific for MLC-2V, no cross-reactivity to MLC-2A.

Control peptide for mouse antibody and rabbit antibody 1
Cat. No. 310-1P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (aa 104-118 of mouse MLC-2V) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Polyclonal rabbit antibody 2
Cat. No. 310 203 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: not tested yet IHC: 1 : 200
Immunogen	Synthetic peptide (aa 3 - 14 of human MLC-2V) coupled to KLH via a C-terminal added cysteine.
Reactivity	Specific for human, no cross reactivity to rat, mouse, pig.
Specificity	Specific for MLC-2V, no cross-reactivity to MLC-2A.

Control peptide for antibody 2
Cat. No. 310-2P	100 µg lyophilized peptide. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized!
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control peptide consists of the synthetic peptide (aa 3 - 14 of human MLC-2V) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.