MLC-2A
A myosin specific for the atrium of the mammalian heart
Fact Sheet
| Monoclonal mouse antibody | |
|---|---|
|
Cat. No. 311 011 IgG, unlabeled |
100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
|
Cat. No. 311 011AT1 purified antibody, ATTO® 647N-labeled |
100 µg purified IgG, lyophilized, fluorescence-labeled with ATTO® 647N. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 100 up to 1 : 2000 (AP staining) 311 011
IP: not tested yet ICC: yes (methanol fixation) 311 011 IHC: 1 : 200 311 011 311 011AT1 |
| Clone | 56F5 |
| Subtype | IgG2b |
| Immunogen | Recombinant protein of human myosin light chain 2A. |
| Reactivity | Human, rat, mouse, no signal in chicken; other species not tested yet. |
| Specificity | Specific for MLC-2A, no cross-reactivity to MLC-2V. |
| Remarks | Protocol for immunohistochemistry of this antibody will be provided with the goods. |
| Control protein | |
| Cat. No. 311-0P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the recombinant protein that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
