MAP 2
A major microtubule associated protein
Fact Sheet
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 188 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 188 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) 188 002 188 003
IP: not tested yet ICC: 1 : 1000 188 002 188 003 IHC: 1 : 250 image |
| Immunogen | Recombinant mouse MAP 2 (aa 2 - 309). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for MAP 2; recognizes all three isoforms. |
| Control protein | |
| Cat. No. 188-0P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the recombinant protein (aa 2 - 309) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
