MAP 2

A major microtubule associated protein

Fact Sheet

Monoclonal mouse antibody
Cat. No. 188 011 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: 1 : 500 image IHC: 1 : 200 image
Clone	198A5
Subtype	IgG1
Immunogen	Recombinant mouse MAP 2 (aa 2 - 309).
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for MAP 2; recognizes all three isoforms.

Polyclonal rabbit antibody
Cat. No. 188 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Cat. No. 188 003 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) 188 002 188 003 IP: not tested yet ICC: 1 : 1000 188 002 188 003 IHC: 1 : 250 up to 1 : 500 (see remarks) 188 002 188 003
Immunogen	Recombinant mouse MAP 2 (aa 2 - 309).
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for MAP 2; recognizes all three isoforms.
Remarks	Works also well on paraffin embedded material.

Polyclonal guinea pig antibody
Cat. No. 188 004 crude antiserum	100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: 1 : 1000 image IHC: 1 : 200 (see remarks) image
Immunogen	Recombinant mouse MAP 2 (aa 2 - 309).
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for MAP 2; recognizes all three isoforms.
Remarks	Works also well on paraffin embedded material.

Control protein
Cat. No. 188-0P	100 µg lyophilized protein. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use.
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control protein consists of the recombinant protein (aa 2 - 309) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.