Liprin-α (Syd 2)

A protein involved in the development of presynaptic active zones

Fact Sheet

Polyclonal rabbit antibody 1
Cat. No. 169 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: not recommended IHC: not recommended
Immunogen	Recombinant protein of mouse liprin-α 3 (aa 313 - 461).
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	This antibody probably cross reacts to isoforms 1, 2, 4, due to sequence homologies.

Control protein for antibody 1
Cat. No. 169-0P	100 µg lyophilized protein. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control protein consists of the antigen (aa 313 - 461) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Polyclonal rabbit antibody 2
Cat. No. 169 102 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: 1 : 500 image IHC: not tested yet
Immunogen	Recombinant protein of mouse liprin-α 3 (aa 463 - 604).
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for liprin-α 3, no cross reaction to isoforms 1, 2, 4.

Control protin for antibody 2
Cat. No. 169-1P	100 µg lyophilized protein. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control protein consists of the antigen (aa 463 - 604) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.