Homer 1 (VesL 1, Syn 47)
A post synaptic density (PSD) protein involved in the targeting of glutamate receptors
Fact Sheet Cat. No. 160 103
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 160 103 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: 1 : 200 up to 1 : 500 (see remarks!) image IHC: not tested yet ELISA: not tested yet |
| Immunogen | Purified recombinant N-terminal parts of Homer 1, 2 and 3. |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | The first 120 aa were used for affinity purification. According to Soloviev et al. (2000), they are homologous in Homer 1 a-d, Homer 2 a-d, and Homer 3 a-d. |
| Remarks | ICC: PFA fixation recommended. Methanol fixation leads to a complete loss of the signal. |
