GFP (Green fluorescent protein)
A universal tool in cell biology
References SySy GFP antibodies
Oelkers JM, Vinzenz M, Nemethova M, Jacob S, Lai FP, Block J, Szczodrak M, Kerkhoff E, Backert S, Schlüter K, Stradal TE, Small JV, Koestler SA & Rottner K (2011). Microtubules as platforms for assaying actin polymerization in vivo. PloS One 6: e19931.
clone 101G4; WB
Koshibu K, Gräff J, Beullens M, Heitz FD, Berchtold D, Russig H, Farinelli M, Bollen M & Mansuy IM (2009). Protein phosphatase 1 regulates the histone code for long-term memory. Journal of Neuroscience 29: 13079-89.
rabbit antibody; IHC
Kaddai V, Gonzalez T, Keslair F, Grémeaux T, Bonnafous S, Gugenheim J, Tran A, Gual P, Le Marchand-Brustel Y & Cormont M (2009). Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte. PLoS one 4(4): e5257.
monoclonal antibodies; WB, ICC
Huang K, Sanders S, Singaraja R, Orban P, Cijsouw T, Arstikaitis P, Yanai A, Hayden MR & El-Husseini A (2009). Neuronal palmitoyl acyl transferases exhibit distinct substrate specificity. FASEB Journal 23: 2605-15.
rabbit antibody; WB, ICC
Zhu GD, Salazar G, Zlatic SA, Fiza B, Doucette MM, Heilman CJ, Levey AI, Faundez V & L'Hernault SW. (2009). SPE-39 family proteins interact with the HOPS complex and function in lysosomal delivery. Molecular Biology of the Cell 20: 1223-40.
rabbit antibody; WB
Saliba RS, Michels G, Jacob TC, Pangalos MN & Moss SJ (2007). Activity-dependent ubiquitination of GABA(A) receptors regulates their accumulation at synaptic sites. Journal of Neuroscience 27: 13341-51.
rabbit antibody; ICC
Trajkovic K, Dhaunchak AS, Goncalves JT, Wenzel D, Schneider A, Bunt G, Nave KA & Simons M (2006). Neuron to glia signaling triggers myelin membrane exocytosis from endosomal storage sites. Journal of Cell Biology 172: 937-48.
rabbit antibody; EM
Jacob TC, Bogdanov YD, Magnus C, Saliba RS, Kittler JT, Haydon PG & Moss SJ (2005). Gephyrin regulates the cell surface dynamics of synaptic GABAA receptors. Journal of Neuroscience 25: 10469-78.
rabbit antibody; ICC
Rotmann A, Strand D, Martiné U, Closs EI. (2004). Protein kinase C activation promotes the internalization of the human cationic amino acid transporter hCAT-1. A new regulatory mechanism for hCAT-1 activity. Journal of Biological Chemistry 279: 54185-92.
rabbit antibody; IP
General references GFP
Zimmer M (2002). Green fluorescent protein (GFP): applications, structure, and related photophysical behavior. Chemical Reviews 102: 759-81.
Review, no abstract available
van Roessel P & Brand AH (2002). Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins. Nature Cell Biology 4: E15-20.
Review
Stephens DJ & Pepperkok R (2001). Illuminating the secretory pathway: when do we need vesicles? Journal of Cell Science 114: 1053-93.
Review
Chamberlain C & Hahn KM (2000). Watching proteins in the wild: fluorescence methods to study protein dynamics in living cells. Traffic 10: 755-62.
Review