GFP (Green fluorescent protein)

A universal tool in cell biology

Fact Sheet

Monoclonal mouse antibody, clone 270F3
Cat. No. 132 011 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: no IP: yes ICC: 1 : 100 IHC: yes
Clone	270F3
Subtype	IgG1
Immunogen	Recombinant full length GFP, glutaraldehyde fixed.
Epitope	QQNTPI
Reactivity	N/A
Specificity	Specific for GFP.
Remarks	Cat. no. 132 111 or 132 002 is recommended for WB.

Monoclonal mouse antibody, clone 101G4
Cat. No. 132 111 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 10 000 (AP staining) blot IP: no ICC: yes (methanol fixated material only) IHC: not tested yet
Clone	101G4
Subtype	IgG2b
Immunogen	Recombinant full length GFP, glutaraldehyde fixed.
Epitope	QQNTPIGDG (aa 183 - 191)
Reactivity	N/A
Specificity	Specific for GFP of jelly-fish.
Remarks	Cat. no. 132 011 or 132 002 is recommended for IP and ICC.

Polyclonal rabbit antibody
Cat. No. 132 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Cat. No. 132 003 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Cat. No. 132 003C3 purified antibody, Oyster^® 550-labeled	50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Oyster^® 550. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex^® (Merck Chemicals) is not compatible with Oyster dyes!
Cat. No. 132 003C5 purified antibody, Oyster^® 650-labeled	50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Oyster^® 650. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex^® (Merck Chemicals) is not compatible with Oyster dyes!
Cat. No. 132 003AT1 purified antibody, ATTO^® 647N-labeled	50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with ATTO^® 647N. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: 1 : 1000 up to 1 : 20000 (AP staining) 132 002 132 003 IP: yes (only serum tested) ICC: 1 : 100 up to 1 : 1000 IHC: yes
Immunogen	Strep-Tag^® fusion protein; full-length GFP.
Reactivity	N/A
Specificity	Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).

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