GFAP

A glial-specific type-III intermediate filament protein

Fact Sheet

Monoclonal mouse antibody, clone 134B1
Cat. No. 173 011 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Cat. No. 173 011BT IgG, biotin-labeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: not recommended IP: yes ICC: 1 : 500 173 011 173 011BT IHC: 1 : 200 (see remarks) 173 011 173 011BT ELISA: yes (see remarks)
Clone	134B1
Subtype	IgG2a
Immunogen	Recombinant full length human GFAP.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for GFAP.
Remarks	IHC: Works also well on paraffin embedded sections. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 173 002 as detector antibody (protocol for sandwich-ELISA).

Monoclonal mouse antibody, clone 64F6
Cat. No. 173 111 IgM, unlabeled	100 µg purified IgM, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 image IHC: 1 : 500 image ELISA: not tested yet
Clone	64F6
Subtype	IgM
Immunogen	Recombinant full length human GFAP.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for GFAP.

Polyclonal rabbit antibody
Cat. No. 173 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 10000 (AP staining) blot IP: yes ICC: 1 : 1000 image IHC: yes (see remarks) image ELISA: yes (see remarks)
Immunogen	Recombinant full length human GFAP.
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for GFAP.
Remarks	IHC: Works also well on paraffin embedded sections. ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 173 011 as capture antibody (protocol for sandwich-ELISA).

Polyclonal guinea pig antibody
Cat. No. 173 004 crude antiserum	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Cat. No. 173 005 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Guinea pig serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) 173 004 173 005 IP: yes ICC: 1 : 500 up to 1 : 1000 173 004 173 005 IHC: 1 : 200 up to 1 : 500 173 004 173 005
Immunogen	Recombinant full length human GFAP.
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for GFAP.

Control protein
Cat. No. 173-0P	100 µg lyophilized protein. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use.
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control protein consists of the recombinant full length human GFAP that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.