GFAP
A glial-specific type-III intermediate filament protein
Fact Sheet Cat. No. 173 211BT
| Monoclonal mouse antibody, clone 186C6 | |
|---|---|
|
Cat. No. 173 211BT IgG, biotin-labeled |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining; see remarks) blot
IP: yes ICC: not recommended IHC: not recommended ELISA: yes (see remarks) |
| Clone | 186C6 |
| Subtype | IgG1 |
| Immunogen | Recombinant full length human GFAP. |
| Reactivity | Human, rat, mouse, cow; other species not tested yet. |
| Specificity | Specific for GFAP. |
| Remarks |
WB: The monoclonal antibodies are less sensitive compared to the polyclonals.
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 173 011 as capture antibody (protocol for sandwich-ELISA). |
