Gephyrin
A protein associated with inhibitory neurotransmitter receptors
Fact Sheet Cat. No. 147 111
| Monoclonal mouse antibody, clone 3B11 | |
|---|---|
|
Cat. No. 147 111 IgG, unlabeled |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 5000 blot
IP: yes ICC: 1 : 1000 image IHC: yes, methanol-acetone fixation ELISA: yes (see remarks) |
| Clone | 3B11 |
| Subtype | IgG1 |
| Immunogen | Purified recombinant doublet of E-Domain (aa 307 - 735). |
| Epitope | C6 domain located in the first half of the E-domain. |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Detects all splice variants that contain a complete E-domain including the C6 domain. |
| Remarks |
This antibody is highly recommended for Western blot experiments and immunoprecipitation. ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 147 003 as detector antibody (protocol for sandwich-ELISA). |
