GADs
Synthesizing GABA, the major inhibitory neurotransmitter in the central nervous system
Fact Sheet
| Polyclonal rabbit antibody 1 against GAD 1 / GAD 67 | |
|---|---|
|
Cat. No. 198 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: not tested yet ICC: 1 : 500 image IHC: not recommended |
| Immunogen | Recombinant mouse GAD 1 (aa 3-101). |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Specific for GAD 1 / GAD 67. |
| Polyclonal rabbit antibody 2 against GAD 1 / GAD 67 | |
|
Cat. No. 198 013 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: not recommended
IP: not tested yet ICC: 1 : 500 image IHC: 1 : 200 up to 1 : 500 image |
| Immunogen | Recombinant mouse GAD 1 (aa 3-101). |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Specific for GAD 1 / GAD 67. |
| Polyclonal rabbit antibody against GAD 2 / GAD 65 | |
|
Cat. No. 198 102 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 198 103 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining; see remarks) 198 103
IP: not tested yet ICC: 1 : 2000 (see remarks) 198 102 IHC: 1 : 1000 (see remarks) 198 102 |
| Immunogen | Purified recombinant mouse GAD 2 (aa 3 - 96). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for GAD 2 / GAD 65. |
| Remarks |
WB: The affinity purified antibody is recommended. ICC/IHC: The crude antiserum is recommended. |
| Polyclonal guinea pig antibody against GAD 2 / GAD 65 | |
|
Cat. No. 198 104 crude antiserum |
100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining; see remarks) blot
IP: not tested yet ICC: 1 : 500 up to 1 : 2000 image IHC: 1 : 500 |
| Immunogen | Purified recombinant mouse GAD 2 (aa 3 - 96). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for GAD 2 / GAD 65. |
| Remarks | WB: The guinea pig antiserum is less sensitive than the rabbit antiserum. |
| Control protein for GAD 2 / GAD 65 | |
| Cat. No. 198-1P | 100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control peptide consists of the recombinant protein (aa 3 - 96) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
